Welcome to Sindh Biotechnologist Association

1.      Introduction Transgenesis is the movement or insertion of a gene from one organism to another organism. The main aim of transgenesis is to develop a transgenic organism. Transgenic organisms have a foreign gene or DNA due to which they exhibit a new trait or character. The whole procedure can be done through physical or biological methods. Following steps are generally involved in transgenesis: 1.1.      Identification of gene of interest The first step is to identify a gene of interest which is coding for a desirable trait. There are many techniques to identify a gene of interest e.g. Gene chip technology. 1.2.      Isolation of the gene The targeted gene must be isolated from an organism. It can be done using chemical treatments. First of all, DNA is cut by the restriction enzyme. The fragments are then separated according to their size using gel electrophoresis. The fragment containing a gene of interest can be identified by a DNA ...

Transgenic animals are commonly known as “Genetically Engineered Organism” (GEO) or “Genetically Modified Organism” (GMO). The advancement in biological science to produce genetically modified animals by introducing a foreign gene into the animal germ-line has raised the strength of bioresearch to an unexpected level (Datta, S., et al, 2018). The transgenic technique gives a very beneficial method to quickly introduce modified genes into the host genome without cross-breeding (Mohammed, F., et al, 2016). The transgenic technology emergence has broadened the development scope in the farm animal’s case and the arrival of new techniques of molecular biology have paved the way by giving the latest aspects to animal breeding ( Rajoriya. R ., et al., 2013). What are transgenic animals? The transgenic animal is a modified animal that carrying a modified gene that has been intentionally admitted into an animal genome. Transgenesis refers to Complementary Deoxyribonucleic Acid (cDNA) inser...