General overview of cell culture
INTRODUCTION: Cell culture refers to the removal of cells from eukaryotic or prokaryotic organisms and grown in a controlled environment. They are grown in controlled conditions to make a cell line which has important applications. The cells can be extracted directly from the tissue and disaggregated by enzymatic or mechanical methods before culture, or they can be generated from an existing cell line or cell strain. Although there are significant variations, mammalian/animal cell culture procedures are fundamentally comparable to those used for bacteria, fungus, and yeast. HISTORY: 1907: 1 st successful tissue culture was made in vitro by the hanging drop technique by Ross Granville 1911: Alexis Carrel and Montrose Burrows defined the term tissue culture and established the first cell line using diluted blood plasma for the media 1920: cell culture formulations were made using compositions of salt solutions 1940: antibiotics were used in cell culture 1960: manufactur...