Nano-pore Sequencing
It is a third-generation sequencing approach by which a single molecule of DNA or RNA can be sequencing without using PCR for amplification or chemical labeling of sample 1 . It was invented by Oxford Nanopore Technologies Ltd. It can easily sequence ultra-long fragments easily and in no time. Working A nano-pore is a pore of nanometer size 10 -12 meters. A nanopore is a protein in nature, embedded in an electrically insulated membrane. An ionic current is passed through the aperture of the pore as the membrane is electrically resistant 2 . A single strand of DNA or RNA when passed through the pore it causes disruption in the current passing through the pore which is then detected 3 . Importance It is important as it is the fastest DNA sequencing technique as it has the capacity of sequencing 50-250 bases per sec/pore. Nanopore technology is used because 4 Ability to sequence single molecules Long read lengths Real-time analysis Cost-effectiveness Versatility Advantage...