Welcome to Sindh Biotechnologist Association

This lecture is presented by our volunteer Zargull Sadein, she is from Karachi Pakistan, and she is covering Gene environment interaction and its impact upon on human health. For video:    For slides:  

Background Using a DNA template, at least one pair of unique primers, deoxyribonucleotides, an effective buffer solution and a thermostable DNA polymerase, the same technique is used to amplify small quantities of DNA as in traditional PCRs. In a thermal cycler containing sensors for measuring the fluorescence of the fluorophore after excitation at the appropriate wavelength, a material marked with a fluorophore is applied to this mixture, enabling the generation rate to be measured for one or more particular items. This makes it possible to calculate the generation rate of the amplified product during each PCR period. The data thus created can be analysed in several samples by computer software to measure relative gene expression (or copy number of the mRNA). In order to assess the occurrence and abundance of a given DNA sequence in these samples, quantitative PCR can also be extended to the identification and quantification of DNA in samples. This calculation is made after each am...

  This presentation will cover mainly Gene Therapy and their subtopics, This presentation is given by Miss Nadia Muzaffar. Video: Slides: Gene Therapy from SindhBiotech

It is a third-generation sequencing approach by which a single molecule of DNA or RNA can be sequencing without using PCR for amplification or chemical labeling of sample 1 . It was invented by Oxford Nanopore Technologies Ltd. It can easily sequence ultra-long fragments easily and in no time. Working A nano-pore is a pore of nanometer size 10 -12 meters. A nanopore is a protein in nature, embedded in an electrically insulated membrane. An ionic current is passed through the aperture of the pore as the membrane is electrically resistant 2 . A single strand of DNA or RNA when passed through the pore it causes disruption in the current passing through the pore which is then detected 3 . Importance It is important as it is the fastest DNA sequencing technique as it has the capacity of sequencing 50-250 bases per sec/pore. Nanopore technology is used because  4 Ability to sequence single molecules Long read lengths Real-time analysis Cost-effectiveness Versatility   Advantage...

This presentation is given by Miss Khunsha Fatima. This presentation will cover mainly Restriction Modification Enzymes, its Types, Applications and its related topics discussed in detail watch the video for more concepts about the topic.  Video: Slides: Restriction Modification Enzymes from SindhBiotech