Welcome to Sindh Biotechnologist Association

Stem cells have been making headlines, sparking curiosity about their potential to revolutionize medical treatments. If you or a loved one have ever pondered the role of stem cells in treating serious diseases, you're not alone. In this article, we'll delve into the world of stem cells, exploring what they are, how they're being used to combat disease and injury, and why they remain at the center of intense debates. Whether you're seeking to understand the basics or considering the possibilities of stem cell therapies, here are the answers to the questions you may have. Progress in the field of stem cell biology and state-of-the-art technologies drives regenerative medicine treatments that aim to guide stubborn non-healing injuries toward complete tissue restoration and the subsequent restoration of their function. A wealth of research has showcased that either coaxing the body's stem cells into action or introducing external stem cell populations to damaged tissues...

This lecture is presented by our volunteer Zargull Sadein, she is from Karachi Pakistan, and she is covering Gene environment interaction and its impact upon on human health. For video:    For slides:  

This video is presented by our volunteer M. Fida ur Rehman, he is from Pakistan, and she is covering ELISA topic. Video:   Slides:  

Background Using a DNA template, at least one pair of unique primers, deoxyribonucleotides, an effective buffer solution and a thermostable DNA polymerase, the same technique is used to amplify small quantities of DNA as in traditional PCRs. In a thermal cycler containing sensors for measuring the fluorescence of the fluorophore after excitation at the appropriate wavelength, a material marked with a fluorophore is applied to this mixture, enabling the generation rate to be measured for one or more particular items. This makes it possible to calculate the generation rate of the amplified product during each PCR period. The data thus created can be analysed in several samples by computer software to measure relative gene expression (or copy number of the mRNA). In order to assess the occurrence and abundance of a given DNA sequence in these samples, quantitative PCR can also be extended to the identification and quantification of DNA in samples. This calculation is made after each am...

  This presentation will cover mainly Gene Therapy and their subtopics, This presentation is given by Miss Nadia Muzaffar. Video: Slides: Gene Therapy from SindhBiotech

It is a third-generation sequencing approach by which a single molecule of DNA or RNA can be sequencing without using PCR for amplification or chemical labeling of sample 1 . It was invented by Oxford Nanopore Technologies Ltd. It can easily sequence ultra-long fragments easily and in no time. Working A nano-pore is a pore of nanometer size 10 -12 meters. A nanopore is a protein in nature, embedded in an electrically insulated membrane. An ionic current is passed through the aperture of the pore as the membrane is electrically resistant 2 . A single strand of DNA or RNA when passed through the pore it causes disruption in the current passing through the pore which is then detected 3 . Importance It is important as it is the fastest DNA sequencing technique as it has the capacity of sequencing 50-250 bases per sec/pore. Nanopore technology is used because  4 Ability to sequence single molecules Long read lengths Real-time analysis Cost-effectiveness Versatility   Advantage...

This presentation is given by Miss Khunsha Fatima. This presentation will cover mainly Restriction Modification Enzymes, its Types, Applications and its related topics discussed in detail watch the video for more concepts about the topic.  Video: Slides: Restriction Modification Enzymes from SindhBiotech

Life is so unpredictable, and every day brings a new challenge for the human race. Along with lots of happiness and cheers, disasters and accidents are also the part and parcel of life. In case of massive disasters such as plane crash, massive fire accidents and marine accidents there is always a deep grief among the masses but the saddest part is that the people could not have access to the remains of their loved ones so that they can perform the funeral rituals according to their religion. But as in case of plane crash the identification of bodies is quite impossible due to high temperature and massive destruction. Usually the identification is carried out by the help of birthmarks, clothing, dental records, fingerprints and jewelry but in such cases when all these methods of identification are not applicable then molecular biology caters the emotional needs of people. DNA typing is the most authentic and sometimes the only available method for identification of human remains in...