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In the realm of ophthalmology, accurate and timely diagnosis is important to ensure optimal patient care. Bacterial keratitis, an infection of the cornea, demands rapid identification for effective treatment. Conventional diagnostic methods have limitations in terms of speed and precision. However, the emergence of Polymerase Chain Reaction (PCR) technology has heralded a new era in ophthalmic diagnostics, offering a revolutionary approach to detecting bacterial keratitis.   Understanding bacterial keratitis: Bacterial keratitis poses a significant threat to ocular health and vision. Caused by bacterial pathogens, this condition can lead to corneal damage and even blindness if left untreated. Swift and accurate identification of the causative bacteria is crucial to administering appropriate antimicrobial therapy. Traditionally, bacterial keratitis diagnosis relied on clinical observation, microbiological cultures, and staining techniques. While these methods are informative, ...

Polymerase Chain Reaction (PCR) is one of the top-most innovation and technique in the field of biotechnology and has aided to the success of different biotechnological and medical research and tests. The PCR technique, developed by Kary Mullis in 1980s, permits scientists to make multiple copies of a target DNA region, simplifying the analysis, and the manipulation of genetic material. A heat-resistant enzyme called DNA polymerase is used for replicating DNA in cycles of heating and cooling. Polymerase chain reaction is an amplification process through which a small amount of nucleic acid (DNA or RNA) can be multiplied and further tests can be performed. The machine in which the process is performed is termed as thermo-cycler and the process of PCR was first introduced by Kary Mullis, a renowned American Biochemist   (Plantsurfer, 2023) . Principles and working of PCR: Polymerase Chain Reaction (PCR) takes place in a series of 3 steps i.e.: Denaturation, Annealing, and El...

Sindh Biotechnologist Association has taken initiative for all young scientists, researchers, and students to have the platform to show their talent and interest in different activities. Topic:  Polymerase chain reaction (PCR) Presentation by: Aymen Arif Research Officer at Halal Food and testing Laboratory, Industrial Analytical Center, H.E.J (ICCBS). PPT of Video Lecture Lecture on Polymerase chain reaction (PCR) from SindhBiotech

  Antibody Testing: Antibodies are the proteins secreted by our immune system in response to an invader that attacks in our body and fights with that infection, for instance, COVID-19. These antibodies are also produced while you inject in a shot of vaccine-like as a vaccine against hepatitis. To clarify, you should keep in mind that the antibodies test is not performed to check the presence of a virus. Instead, it is used to monitor your body's immune system, either it is working against the infection or not. This test is also known as a serology test . Role of IgM & IgG Both types of antibodies are essential to measuring the exposure of the viral infection. In the case of SARS- CoV-2, doctors look for IgG antibodies are found abundantly in our blood and develop after the immune system has covered the infection. IgM antibodies , the first antibody formed when a new infection occurs and fight against it, develop early while an infection occurs. It takes...